Vladimir Polshakov's CV

Vladimir I. Polshakov

Curriculum Vitae

Leading Scientist
Center for Drug Chemistry

7, Zubovskaya str.
Moscow, 119815
Russia

Phone and Fax numbers:
Tel: +7(095) 246-6985
Fax: +7(095) 2467805

Societies/Awards	Public Lectures	Publications

Academic History

1976-81	B.Sc., Chemistry, Chemical Department, Moscow State University
1981-84	Junior Researcher, Center for Drug Chemistry, Moscow
1981-85	Ph.D., Organic Chemistry, Center for Drug Chemistry, Moscow
1985-92	Researcher, Center for Drug Chemistry, Moscow
1989-90	Post-doctoral training, National Institute for Medical Research, London
1992-94	Postdoctoral Fellow (Wellcome Trust Fellowship), National Institute for Medical Research, Mill Hill, London
2000	D.Sc. (Biochemistry), Moscow State University
1995-01	Senior Researcher, Center for Drug Chemistry, Moscow
2001+	Leading Scientist, Center for Drug Chemistry, Moscow
2004+	Leading Scientist, Center for Magnetic Tomography and Spectroscopy, Moscow State University

Scientific Awards

1992 - 1994 Wellcome Trust International Fellowship
1995 - 2000 Howard Hughes Medical Institute International Scholarship
1997 - 1998 Russian Foundation for Basic Research Grant
1999 - 2001 Russian Foundation for Basic Research Grant
2000 - 2003 Wellcome Trust Collaborative Research Initiative Grant
2002 - 2004 Russian Foundation for Basic Research Grant
2000 - 2005 Howard Hughes Medical Institute International Scholarship
2005 - 2007 Russian Foundation for Basic Research Grant
2008 - 2010 Russian Foundation for Basic Research Grant

Scientific Societies Membership

1981+ All-Russian Mendeleev Chemical Society
1996 - 1998 New York Academy of Sciences
2000+ American Association for the Advancement of Science
2001+ American Chemical Society

Academic Activities

2006 + Editor, Central European Journal of Medicine

Public Lectures and Seminars

Presented more than 30 plenary lectures at Russian and International Meetings.

Scientific Publications

2008

Ivanova, E.V., Alkalaeva, E.Z., Birdsall B., Kolosov, P.M., Polshakov, V.I and Kisselev, L.L. (2008) Interface of the Interaction of the middle domain of human translation termination factor eRF1 with eukaryotic Ribosomes, Mol. Biol. (Rus)., 42: 1056-1066.
Vostrikov, V.V., Selishcheva, A.A., Sorokoumova, G.M., Shakina, Y.N., Shvets, V.I., Savel'ev, O.Y. and Polshakov, V.I. (2008) Distribution coefficient of rifabutin in liposome/water system as measured by different methods, Eur. J. Pharm. Biopharm., 68: 400-405.

2007

Ivanova, E.V., Kolosov, P.M., Birdsall, B., Kelly, G., Pastore, A., Kisselev, L.L. and Polshakov, V.I. (2007) Eukaryotic class-1 translation termination factor eRF1: the NMR structure of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis, FEBS Journal, 274:4223-37.
Kovalevskaya, N.V., Smurnyy, Y.D., Birdsall, B., Feeney, J. and Polshakov, V.I. (2007) Structure factors that determine selectivity of binding of antibacterial trimethoprim with dihydrofolate reductase, Khim-Pharm. Zh. (Rus), #7: 8-11.
Polshakov, V.I.(2007) NMR spectroscopy methods in drug design, New of NMR in Letters (Rus, Kazan), 1782-1798.
Mantsyzov, A.B., Ivanova, E.V., Birdsall, B., Kolosov, P.M., Kisselev, L.L. and Polshakov, V.I. (2007) NMR assignments of the C-terminal domain of human polypeptide release factor eRF1, Biomol. NMR Assign., 1:183-185.
Brandt, N.N., Brovko, O.O., Chikishev, A.Yu., Itoh, K., Lebedenko, S.I., Polshakov V.I. and Sakodynskaya, I.K. (2007) Laser control of the structure of a photosensitive substrate for enzymatic reaction, Laser Physics, 17: 1262-1265

2006

Polshakov, V.I., Birdsall, B. and Feeney, J. (2006) Effects of co-operative ligand binding on protein amide NH hydrogen exchange, J. Mol. Biol., 356: 886-903.
Ivanova, E.V., Kolosov, P.M., Birsall, B., Kisselev, L.L. amd Polshakov, V.I. (2006) NMR assignments of the middle domain of human polypeptide release factor eRF1, J. Biomolecular NMR, 36, Suppl.1: 8.

2005

Kovalevskaya, N.V., Smurnyy, Y.D., Polshakov, V.I., Birdsall, B., Bradbury, A.F., Frenkiel, T. and Feeney, J. (2005) Solution structure of human dihydrofolate reductase in its complex with trimethoprim and NADPH, J. Biomolecular NMR, 33: 69-72.

2003

Polshakov, V.I.(2003) NMR spectroscopy in studies of protein structure: second wind, New of NMR in Letters (Rus, Kazan), #1-2: 1292-1307.

2002

Polshakov, V.I., Biekofsky, R.R., Birdsall, B. and Feeney, J. (2002) Towards understanding the origins of the different specificities of binding the reduced (NADPH) and oxidised (NADP⁺) forms of nicotinamide adenine dinucleotide phosphate coenzyme to dihydrofolate reductase, J. Mol. Struct., 602-603: 257-267.
Polshakov, V.I., Smirnov, E.G., Birdsall, B., Kelly, G. and Feeney, J. (2002) NMR-based solution structure of the complex of Lactobacillus casei dihydrofolate reductase with trimethoprim and NADPH, J. Biomolecular NMR, 24: 67-70.

2001

Polshakov V.I. (2001) Dihydrofolate reductase: structure aspects of mechanisms of enzyme catalysis and inhibition, Russian Chemical Bulletin, 50: 1652 - 1669.

2000

Birdsall, B., Polshakov, V.I. and Feeney J. (2000) NMR studies of ligand carboxylate group interactions with arginine residues in complexes of Lactobacillus casei dihydrofolate reductase with substrates and substrate analogues, Biochemistry, 39 : 9819-9825.

1999

Polshakov, V.I., Birdsall, B., Frenkiel, T.A., Gargaro, A.R. and Feeney, J. (1999) Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophylic antifolate drug trimetrexate, Protein Sci., 8: 467-481.
Polshakov, V.I., Morgan, W.D., Birdsall, B. and Feeney J. (1999) Validation of a new restraint docking method for solution structure determination of protein-ligand complexes, J. Biomolecular NMR, 14: 115-122.
Polshakov, V.I., Birdsall, B. and Feeney J. (1999) Characterisation of rates of ring-flipping in trimethoprim in its ternary complexes with Lactobacillus casei dihydrofolate reductase and coenzyme analogues, Biochemistry, 38: 15962-15969.

1998

Gargaro, A.R., Soteriou, A., Frenkiel, T.A., Bauer, C.J., Birdsall, B., Polshakov, V.I., Barsukov, I.L., Roberts, G.C.K. and Feeney J. (1998) The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate. J. Mol. Biol., 277: 119-134.

1997

Polshakov, V.I., Williams, M.A., Gargaro, A.R., Frenkiel, T.A., Westley, B.R., Chadwick, M.P., May, F.E.B. and Feeney, J. (1997) High-resolution solution structure of human pNR-2/pS2: a single trefoil motif protein. J. Mol. Biol., 267: 418-432

1996

Gargaro, A.R., Frenkiel, T.A., Nieto, P.M., Birdsall, B., Polshakov, V.I., Morgan W.D. and Feeney, J. (1996) NMR detection of arginine-ligand interactions in complexes of Lactobacillus casei dihydrofolate reductase. Eur. J. Biochemistry 238: 435-439.
Anisimova, O.S., Lopatin, A.V., Chistyakov, V.V., Polshakov, V.I., Soloveva, N.P., Galushko, A.V., Pleshakova, A.P., and Sheinker, Yu.N. (1996) Study of methabolism of incazan by methods of chromatography, NMR and mass-spectrometry. Khim-Pharm. Zh., 30 (3): 3-6 (Rus).
Anisimova, O.S., Lopatin, A.V., Polshakov, V.I., Chistyakov, V.V., Ermachenkov, I.A., Golovanova, I.V., Ivanov, P.Yu., Pleshkova, A.P. and Sheinker, Yu.N. (1996) Study of pirlindole methabolism. Khim-Pharm. Zh., 30 (5): 15-17 (Rus).

1995

Polshakov, V.I., Frenkiel, T.A., Birdsall, B., Soteriou, A. and Feeney J. (1995) Determination of stereospecific assignments, torsion-angle constraints and rotamer populations in proteins using the program AngleSearch. J. Magn. Res., Ser. B., 108: 31-43.
Polshakov, V.I., Birdsall, B., Gradwell, M.J. and Feeney J. (1995) The use of PM3 SCF MO quantum mechanical calculations to refine NMR-determined structures of complexes of antifolate drugs with dihydrofolate reductase in solution. J. Molecul. Struct. (Theochem), 357: 207-216.
Morgan, W.D., Birdsall, B., Polshakov, V.I., Sali, D. Kompis, I. and Feeney J. (1995) Solution structure of a brodimoprim analogue in its complex with Lactobacillus casei dihydrofolate reductase. Biochemistry, 34: 11690-11702.
Polshakov, V.I., Frenkiel, T.A., Westley, B., Chadwick, M., May, F., Carr, M.D. and Feeney J. (1995) NMR-based structural studies of the pNR-2/pS2 single domain trefoil peptide. Similarities to porcine spasmolytic peptide and evidence for monomeric structure. Eur. J. Biochem., 233: 847-855.

1994

Polshakov, V.I. and Feeney J. (1994) AngleSearch v. 2.10. Conformational grid search program from the NMRTABLE package for obtaining stereospecific assignments, torsion angle constraints and rotamer populations. The user guide. NIMR, London, England.
Martorell, G., Gradwell, M.J., Birdsall, B., Bauer, C.J., Frenkiel, T.A., Cheung, H.T.A., Polshakov, V.I., Kuyper, L. and Feeney J. (1994) Solution structure of bound trimethoprim in its complex with Lactobacillus casei dihydrofolate reductase. Biochemistry, 33: 12416-12426

1993

Chistyakov, V.V., Anisimova, O.S., Polshakov, V.I., Ermachenkov, I.A. and Sheinker, Yu. N. (1993) Methabolism of tetrindol. Khim-Pharm. Zh., 27(1): 4-6 (Rus).
Maslova, M.M., Marchenko, N.B., Polshakov, V.I., and Glushkov, R.G. (1993) New method for synthesis of 3-nitroquinolone-4 derivatives. Khim-Pharm. Zh., 27(2): 56-60 (Rus).

1992

Velezheva, V.S., Melman, A.I., Polshakov, V.I. and Anisimova, O.S. (1992) A novel synthesis of 2-aryl-3-oxy(alkoxy)-4-quinolones expanding the ring of 1-acetyl-2-arylmethylen-3-indolines. Khim. Geterocycl. Soedin., #2: 279-280 (Rus).
Velezheva, V.S., Ryabova, S.Y., Melman, A.I. and Polshakov, V.I. (1992) 1-acetyl-2-chloro-3-iminoindoline hydrochloride and its N-acetyl derivatives in nucleophilic-substitution reactions. Khim. Geterocycl. Soedin., #1: 49-54 (Rus).

1991

Carr, M.D., Birdsall, B., Frenkiel, T.A., Bauer, C.J., Jimenex-Barbero, J., Polshakov, V.I., McCormic, J.E., Roberts, G.C.K. and Feeney, J. (1991) Dihydrofolate reductase: sequential resonance assignments using 2D and 3D NMR and secondary structure determination in solution. Biochemistry, 30: 6330-6341.
Burova, O.A., Smirnova, N.M., Polshakov, V.I., Chernov, G.S., Losev, G.A. and Safonova, T.S. (1991) Pyrido[2-3-d]pyrimidines. 4. Synthesis and some conversions of oxo(hydrohy)pyrido[2,3-d]pyrimidines. Khim. Geterocycl. Soedin., #5: 674-680 (Rus).
Glushkov, R.G., Maslova, M.M., Marchenko, N.B. and Polshakov, V.I. (1991) A new method for producing a-nitroacetophenones. Khim-Pharm. Zh., 25 (3): 61-62 (Rus).

1990

Velezheva, V.S., Melman, A.I., Smushkevich, Yu.I., Polshakov, V.I. and Anisimova, O.S. (1990) 1-Acetyl-2-bromine-3-indolinone in the reactions of nucleophilic-substitution and synthesis of pyrollo[3,2-b]indoles. Khim-Pharm. Zh., 24(12): 46-51 (Rus).

1989

Polshakov, V.I. and Sheinker, Yu.N. (1990) Interaction of spirazidine, prospidine, and spirobromine with nucleic-acid components. Khim-Pharm. Zh., 23 (1): 10-16 (Rus).

1988

Polshakov, V.I., Sheinker, Yu.N. and Chernov, V.A. (1988) Spirazidine conversion in aqueous media. Khim-Pharm. Zh., 22 (12): 1413-1417 (Rus).
Olshanskaya, O.V., Polshakov, V.I., Presnova, Zh.D., Chernov, V.A., Safonova, T.S. and Shteinpress, A.B. (1988) Khim-Pharm. Zh., 22, (8): 905-907 (Rus).

1987

Levshin, I.B., Chistyakov, V.V., Polshakov, V.I. and Sheinker, Yu.N. (1988) 2-Allylaminothiazoline-4-one in acylation reaction. Khim. Geterocycl. Soedin., #8: 1135-1140 (Rus).
Polshakov, V.I., Dvoryantseva, G.G., Sheinker, Yu.N. and Safonova, T.S. (1987) Interaction of antitumorous drugs prospidine and spirobromine with nucleotides. Khim-Pharm. Zh., 21 (5): 528-536 (Rus).

1986

Polshakov, V.I., Dvoryantseva, G.G., Sheinker, Yu.N., Presnova, Zh.F., Chernov, V.A. and Safonova, T.S. (1986) Investigation of ways of spirobromine biotransformation. Khim-Pharm. Zh., 20 (8): 905-911 (Rus).
Dvoryantseva, G.G., Tetenchuk, K.P., Polshakov, V.I. and Elina, A.S. (1986) Photochemical reactions of biologically important N-oxides of quinoxalines. Khim-Pharm. Zh., 20 (4): 399-408 (Rus).
Musatova, I.S., Polshakov, V.I., Dvoryantseva, G.G., Chistyakov, V.V. and Elina, A.S. (1985) The synthesis and properties of 4-oxo-3-dihydrobenzo(g)-pteridine-2-carboxylic acid and derivatives. Khim-Pharm. Zh., 20 (2): 182-187 (Rus).

1985

Polshakov, V.I., Dvoryantseva, G.G., Sheinker, Yu.N., Chernov, V.A. and Safonova, T.S. (1985) Study of the alkylating ability of prospidin in model reactions with organic phosphates. Khim-Pharm. Zh., 19 (6): 661-667 (Rus).
Dvoryantseva, G.G., Polshakov, V.I., Sheinker, Yu.N., Shvedov, V.I., Grynev, A.N., Mashkovsky, M.D., Karapetyan, H.A.and Struchkov, Yu.T. (1985) Structure-activity relationships in the pyrazino[3,3,1-j,k]-carbazole derivatives. A new antidepressant pirlindole and related compounds. Eur.J.Med. Chem., 20: 414-418.

1984

Polshakov, V.I., Dvoryantseva, G.G., Sheinker, Yu.N., Safonova, T.S. and Chernov, V.A. (1984) The study of transformations of the new antineoplastic drug prospidin in aqueous-media. Khim-Pharm. Zh., 18 (8): 912-919 (Rus).

Academic History	Societies/Awards	Talks/Seminars